Table 1 Summary of In vitro studies and results
Author, year, Country | Cell line | Isoform | Results |
|---|---|---|---|
Gao, T. 2016, China | SW480 | DCLK1-L/S | High expression of DCLK1 led to: Promote the migration and invasion Decrease in E cadherin expression, however enhanced the expression of Vimentin and ZEB1, which are mesenchymal markers |
O'Connell, M. R. 2015, USA | HEK293 and HCT116 | DCLK1-S | Identify specific functions of DCLK1 isoforms: Proposed an alternative β-promoter activator, known as NF-κBp65, that aids us in preventing colon cancer by DCLK1-S, which might be a critical target |
Roy, B. C. 2019, USA | HCT116 and SW480 | DCLK1-L/S | Examined the presence of autophagy: Increased DCLK1 promoter activity followed by co-localization of p62/Dclk1, which is led to inhibit DCLK1’s removal |
Sarkar, S. 2017, USA | HEK293, HCT116, COLO-205, RKO, COLO-320, SW1417, CCD841 | DCLK1-S | High expression of DCLK1-S resulted in decreased FOXD3 that led to increase invasive potential in cells |
Sarkar, S. 2017, USA | HEK293, HCT116, COLO-205 | DCLK1-S | Generating PS41014 antibody, an anti DCLK1-S antibody, which could be used to assess CRC risk |
Suehiro, Y. 2018, Japan | COLO-320 | DCLK1-L | Proposed combination of LRRK, a DCLK1 inhibitor, and 5-FU as a potential treatment |
Vedeld, H. M. 2014, Norway | 6 bile duct, 4 urinary bladder, 8 breast, 19 colon, 2 gall bladder, 4 gastric, 4 kidneys, 3 leukemia, 4 lung, 1 MPNST, 4 ovarian, 6 pancreatic, 1 prostate, 4 testis, and 4 uterus cancer cell lines | DCLK1-L | Established that hypermethylation of DCLK1 promoter could be an early detection biomarker in CRC |
Venugopal, A. 2017, USA | HCT 116 and DLD-1 | N/A | Promote self-renewal ability of CRC cells |
Weygant, N. 2016, USA | SW480 | DCLK1-L | Upregulation of DCLK1, promote EMT of CRC cells as determined |
Weygant, N. 2014, USA | HCT116, HT-29, and DLD-1 | DCLK1-L/S | Inhibition DCLK1 by LRRK2-IN-1 LRRK2-IN-1 inhibits proliferation DCLK1 promotes resistance to LRRK2-IN-1 |
Westphalen, C. B. 2014, USA | SW105 R26-Tom, R26-TGFP, or R26-DTA mice | DCLK1-L | Promote quiescence and tumor initiating in CRC cells |
Makino, S. 2020 Japan | SW480, HCT116 | DCLK1-L/S | Detecting high expression of DCLK1: Established the invasion and migration role of DCLK1 in CRC cells as determined DCLK1 upregulation induce EMT in CRC cells Found the positive correlation between DCLK1 and TRIB3 Found the effectiveness of combination therapy L-OHP with LRRK2 |
Mohammadi, Y. 2018 Iran | SW-48 and HCT-116 | N/A | High expression of DCLK1 promote invasion and migration and decrease apoptosis and sphere forming ability in CRC cells |
Chandrakesan, P. 2015, USA | ApcMin/+ mice IEC | N/A | Promote self-renewal ability of CRC cells |
Chandrakesan, P. 2014, USA | ApcMin/+ mice IEC | N/A | Established that DCLK1 upregulation promote EMT, pluripotency, and self-renewal ability of CRC cells |
Sureban, S. M. 2020, USA | HT29, HCT116, and LoVo | DCLK1-L/S | Application of DCLK1 as a molecular target in monoclonal antibody-based CAR-T cell therapy, which could be induce cytotoxicity Suggesting that high surface expression of DCLK1 is associated with increased cologenic capacity of CRC cells |
Sureban, S. M. 2011, USA | HCT-116 | DCLK1-L | Application of DCLK1 as a molecular target in nanoparticle (NP)-siDCAMKL-1therapy |
Wu, X. 2016, China | SW480 and SW620 | DCLK1-L | Establishing the combination therapy of 5-FU with XAV939 led to downregulation of DCLK1 in CRC cells |
Dai, T. 2018, China | NCM460 and HCT116 | DCLK1-L DCLK1-S | Isoform specific function of DCLK1 by generating monoclonal antibodies |
Mohammadi, C. 2021, Iran | HCT-116 | N/A | High expression of DCLK1 promote EMT and cell proliferation, decreased apoptosis |
Park, S. Y. 2019, Korea | HCT116, HT29, SW480 | DCLK1-L DCLK1-S | Induce cancer stemness by confining the LEF1/DCLK1 axis through niclosamide |
May, R. 2009, USA | SW480 | DCLK1-L | Introduce DCLK1 as a cell surface marker |
Li, L. 2019, USA | HCT116, WT | N/A | DCLK1 upregulation induce chemoresistance with 5-FU treatment by inhibiting apoptosis activity |
Li, L. 2013, USA | HCT116 | DCLK1-L | Induce apoptosis and self-renewal ability of CRC cells by FACS, ELDA, qRT-PCR |
Lu, Y. 2018, Japan | HCT116, HEK293FT, H1299, MCF10A | N/A | Dclk1 induce DNA damage assessed DCLK1 promotes cell proliferation |