Fig. 3
From: GRB10 is a novel oncogene associated with cell proliferation and prognosis in glioma
Knockdown of GRB10 inhibits glioma cell growth and colony formation. a, b The WB was conducted to determine the level of GRB10 in U251(a) and SHG44 (b) cells stably knockdown of GRB10. c, d The cell viability detected by MTS assay in U251 (c) and SHG44 (d) cells stably knockdown of GRB10. e–h EdU was performed to determine the cell viability in U251 (e–f) and SHG44 (g–h) cells stably knockdown of GRB10. i–l Colony-formation assay of U251 and SHG44 cells with stably knockdown of GRB10 (i, j), colony number counted by Image J (k, l). Data are analyzed by a two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001