Fig. 2

LINC00893 functions as a tumor suppressor factor to inhibit the proliferation, migration, invasion and EMT of PCa cells. PC-3 and LNCaP cells were transfected with pcDNA3.1-LINC00893 plasmids or pcDNA3.1-vector for 48 h. A LINC00893 expression level was examined by RT- qPCR in cells transfected with pcDNA3.1-LINC00893 or vector. B CCK-8 proliferation assay in cells transfected with pcDNA3.1-LINC00893 or vector was perfromed at indicated time points. C EdU incorporation assay in cells transfected with pcDNA3.1-LINC00893 or vector. Scale bar: 50 μM. D Colony formation assay in cells transfected with pcDNA3.1-LINC00893 or vector. E, F Transwell migration assay (E) and invasion assay (F) in cells transfected with pcDNA3.1-LINC00893 or vector. G The protein levels of E-cadherin and mesenchymal markers N-cadherin and vimentin were assessed by Western blot in cells transfected with pcDNA3.1-LINC00893 or vector. Three independent assays were performed with three technical replicates. The error bars are defined as s.d. *, P < 0.05, **, P < 0.01, and ***, P < 0.001