Fig. 6

SOCS3 silencing attenuated the effects of miR-3173-5p inhibitor in PCa cells. A The knockdown efficiency of SOCS3 siRNA in PC-3 and LNCaP cells was examined by Western blot. For B-G, PC-3 and LNCaP cells were transfected with NC inhibitor, miR-3173-5p inhibitor or miR-3173-5p inhibtor + si-SOCS3. B, C The proliferation ability of cells in the above experimental groups was evaluated by CCK-8 (B) and EdU incorporation assay (C). D Colony formation assay in PC-3 and LNCaP cells of the above experimental groups. Transwell migration assay (E) and invasion assay (F) in PC-3 and LNCaP cells of the above experimental groups. G The protein levels of EMT markers E-cadherin, N-cadherin and vimentin in different treatment groups were detected by Western blot. Three independent assays were performed with three technical replicates. The error bars are defined as s.d. *, P < 0.05, **, P < 0.01, and ***, P < 0.001