Fig. 8

Overexpression of LINC00893 suppresses the tumorigenesis of PCa cells in nude mice. PC-3 cells stably expressing LINC00893 (PC-3-LINC00893) or the control cells (PC-3-vector) were inoculated subcutaneously into NOD/SCID mice (n = 6 in each group). A The volume of the xenograft tumor was measured every 5 days. B The weight of the subcutaneous xenograft tumor was measured on day 30. C LINC00893 and miR-3173-5p expression levels in xenograft tumor tissues were assessed by RT-qPCR. D The protein levels of SOCS3, E-cadherin, vimentin and N-cadherin in xenograft tumor tissues was detected by Western blot. E Ki-67 and SOCS3 protein expression levels in xenograft tumor sections were detected by IHC staining: Scale bar: 100 μM. Three independent assays were performed with three technical replicates. The error bars are defined as s.d. *, P < 0.05, **, P < 0.01, and ***, P < 0.001