Fig. 4

VEGF-B and AhR expression after treatment with TCDD or SR1. A CSLCs were treated with or without TCDD and SR1 for 24 h and VEGF-B and AhR mRNA expression was analyzed via reverse transcription-quantitative PCR. B Protein expression levels of VEGF-B, AhR, E-cadherin and vimentin were analyzed via western blotting. The protein expression was normalized to GAPDH. C Fold change of VEGF-B, AhR, E-cadherin and vimentin protein expression. The results are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. AhR aryl hydrocarbon receptor, TCDD 2,3,7,8-tetrachlorodibenzo-p-dioxin, SR1 StemRegenin 1, CSLC choriocarcinoma stem-like cells