Fig. 2

Trehalose induces macropinocytosis in U373-MG cells. Cells were treated with 90 mM trehalose for 48 h, and fluorescein-conjugated Dextran 70 kDa (FITC-Dex) was added at the final 24 h (A, C) or at the final 1 h (B); representative images of fixed cells observed by fluorescence microscopy (A), by fluorescence microscopy merged with bright-field illumination (B), or by fluorescence microscopy of DAPI-stained cells to visualize nuclei (C). D Cells were treated with 90 mM trehalose for 3 h, and concomitantly labelled with FITC-Dex and intracellular acidic compartment probe LysoTracker Red; representative images of live cells observed by fluorescence microscopy. Images derive from at least 3 independent experiments. Scale bar = 10 mm