Fig. 4
From: The autophagy inducer trehalose stimulates macropinocytosis in NF1-deficient glioblastoma cells
Trehalose induces autophagy to a different extent in glioblastoma U373-MG and T98G cells. U373-MG (A) and T98G (B) cells were untreated (C) or treated with 90 mM trehalose (TRE) for different experimental times. Autophagy was evaluated by the ratio LC3-II/LC3-I, which reliably evaluates the amount of autophagosomes. Results are presented as mean ± SE of 3 to 8 independent experiments (A), or 3 to 4 independent experiments (B). *p < 0.05, **p < 0.01, and ***p < 0.001, significantly different from control cells at the corresponding experimental time. Typical western blots are shown