Fig. 6
From: The autophagy inducer trehalose stimulates macropinocytosis in NF1-deficient glioblastoma cells
EIPA increases trehalose-induced autophagy in U373-MG cells. Cells were untreated (C), or treated with 25 mM EIPA (EIPA), 90 mM trehalose alone (TRE) or combined with EIPA (TRE + EIPA), for 48 or 72 h. A Autophagy was evaluated by western blot as the ratio LC3-II/LC3-I. Results are presented as mean ± SE of 4 to 7 independent experiments. *p < 0.05, significantly different from trehalose-treated cells. Typical western blots are shown. B Cells were cotreated with 30 mM chloroquine (CQ) for the final 6 h. Western blots derive from 2 independent experiments performed in duplicates, and numbers refer to the western blot above. Autophagy was evaluated as ratio LC3-II/LC3-I; LC3-II levels are also reported (as arbitrary units normalized by Ponceau S membrane staining)