Fig. 7
From: The autophagy inducer trehalose stimulates macropinocytosis in NF1-deficient glioblastoma cells
Treahalose-induced macropinocytosis in U373-MG cells is inhibited by EIPA. Cells were untreated (C), or treated for 48 h with 25 mM EIPA (EIPA), 90 mM trehalose alone (TRE) or combined with EIPA (TRE + EIPA); FITC-dextran 70 kDa (FITC-Dex) probe was added for the final 3 h. A The mean fluorescence intensity (columns) is reported as mean percentage (over control cells) ± SE, and the percentage of fluorescent cells (line) is reported as mean ± SE of 3 independent experiments performed in replicates. ***p < 0.001, significantly different from trehalose-treated cells. Representative histogram plots of FITC-Dex-labelled cells are shown. B is blank sample (cells without probe). B Phase-contrast microscopy images of live U373-MG cells treated with trehalose with or without EIPA. Images derive from 2 independent experiments. Scale bar = 50 mm