CRISPR/Cas9 system in breast cancer therapy: advancement, limitations and future scope

Table 1 Recent studies highlighting different altered gene using CRISPR/Cas9 for BC therapy

Target Gene	Cell line	CRISPR approach	Effects	References
MYC Oncogene	–	CRISPR/Cas9-mediated mutagenesis	Decreased MYC expression and cell proliferation	[23]
CXCR7 and CXCR4	MDA-MB-231	CRISPR/Cas9 knockout	decreased tumor cell proliferation, invasion, and tumor growth	[26]
PTEN	SUM159	CRISPR activation	Lowers cancer aggressiveness	[27]
miRNA23b and miR27b	MCF-7	CRISPR/Cas9 knockout	Decreased tumor growth	[29]
MASTL	Human mammary tumor cell lines	CRISPR-based interruption	Restricts cell proliferation	[37]
FASN	MCF-7	Type 2 CRISPR/Cas9	Inhibits cell proliferation, survival,growth, cell cycle, migration, cell adhesion, and DNA replication	[39]
CDK7	TNBC cell lines	CRISPR/Cas9 genetic editing	Inhibits cell growth and tumorigenesis	[44]

ISSN: 1475-2867