Fig. 2

MiR-320c is targeted by circPDSS1 and miR-320c overexpression inhibits BC cell malignant behaviors. A, B Subcellular fractionation and FISH assays were implemented to determine the distribution of circPDSS1 in the nuclei and cytoplasm of BC cells. C RNA pull down assay measured the RNA enrichment of 8 miRNAs screened out through starBase that possibly bind to circPDSS1. D The expression of miR-320c in BC cells transfected with miR-320c mimics was analyzed via qRT-PCR. E Luciferase reporter assay was applied for confirming the binding sites between circPDSS1 and miR-320c acquired from starBase. F MiR-320c expression in BC cells and mammary epithelial cells was measured via qRT-PCR. G, H CCK-8 and colony formation assays were implemented to measure the proliferation of the transfected BC cells. I TUNEL assay was used to test the apoptosis of BC cells upon miR-320c overexpression. J, L Transwell, wound healing and IF assays were conducted to examine the invasion, migration and EMT of the transfected BC cells. ^**P < 0.01