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Fig. 2 | Cancer Cell International

Fig. 2

From: Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer

Fig. 2

Impact of menin silencing on proliferation and transcriptome of OC cells. Changes of MEN1 mRNA relative abundance (A), menin protein expression (B) and cell proliferation (C) determined by RT-qPCR, western blot and MTT assay, respectively, in PEO1 (left) and PEO4 (right) cells 96 h post transfection with three siRNAs targeting different regions of the MEN1 mRNA. Scramble siRNA (CTRL) was used as the negative control. Error bars represent the mean of replicate values ± SD (* p ≤ 0.05). (D) Venn diagrams showing the number of down- (left) and up- (right) regulated transcripts in PEO1 and PEO4 cells 96 h post transfection with MEN1-targeting siRNA #3. RNA-seq was performed in biological duplicates. (E) Graphic representation of determined by IPA statistically significant pathways, concordantly deregulated upon MEN1 silencing in both PEO1 (blue) and PEO4 (red) cells. The dashed orange line marks the Benjamini Hochberg p-value (B-H) threshold (0.05). (F) Gene set enrichment analysis (GSEA) showing MYC and DNA repair GO terms highlighted by genes, deregulated upon MEN1 silencing in PEO1 and PEO4 cells. Negative and positive Normalized Enrichment Scores (NES) indicate that down- and up-regulated genes are over-represented. FDR stands for False Discovery Rate

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Cancer Cell International

ISSN: 1475-2867

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