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Fig. 3 | Cancer Cell International

Fig. 3

From: Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer

Fig. 3

Effects of menin pharmacological inhibition on OC cells proliferation and transcriptome. (A) PEO1 (left) and PEO4 (right) relative cell viability assessed in cells treated with increasing concentrations of MI-136. Data represent the mean of six independent replicates ± SD (* p ≤ 0.05). (B) Venn diagrams showing the number of down- (left) and up- (right) regulated transcripts in PEO1 and PEO4 cells, cultivated in the presence of 0.8 µM MI-136 or vehicle (DMSO) for 9 days. First quartile (Q1) fold-change threshold was applied to identify differentially expressed genes (|FC| ≥ Q1, padj ≤ 0.05). RNA-seq was performed in biological triplicates. (C) Heatmap and hierarchical clustering with dissimilarity measured using Manhattan distance showing common concordantly deregulated transcripts in PEO1 and PEO4 cells upon MEN1 silencing or pharmacological inhibition with 0.8 µM MI-136 (|FC| ≥ Q1, padj ≤ 0.05). (D) Circos plot showing genes, over- (red) and under- (blue) rerepresented in MI-136-treated respect to control (DMSO-treated) PEO4 cells, and the statistically significant (p ≤ 0.05) aryl hydrocarbon receptor (AhR) pathway and integrin signaling pathway that tend to be up-regulated

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Cancer Cell International

ISSN: 1475-2867

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