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Fig. 2 | Cancer Cell International

Fig. 2

From: Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint

Fig. 2

LINC00460 knockdown inhibits the malignant characteristics of PANC-1 cells in vitro and suppresses the growth of tumor xenograft in vivo. A The expression of LINC00460 in PANC-1 cells was detected by qRT-PCR. B The viability (OD450) of PANC-1 cells was measured by MTT assay. C The proliferation of PANC-1 cells was determined by EdU assay (200 ×). D The apoptosis of PANC-1 cells was analyzed by flow cytometry assay. (E) The migration ability of PANC-1 cells was measured by transwell assay. F The invasion ability of PANC-1 cells was measured by transwell assay. G The protein levels of E-cadherin and N-cadherin were determined by Western blot. H The images of tumor xenograft with LINC00460 knockdown. I The tumor volumes were monitored at different time points. J The tumor weights were measured 4 weeks later. (K) Ki67 staining in PC tissues was measured by IHC assay (400 ×). **P < 0.01 vs. the sh-NC group

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