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Fig. 5 | Cancer Cell International

Fig. 5

From: Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint

Fig. 5

Identification of ANLN as the target gene of miR-503-5p. A Three target genes of miR-503-5p from databases (TargetScan, miRDB and TCGA) were exhibited by Venn diagram. B The predicted complementary binding site of miR-503-5p and ANLN. C The luciferase activity in PANC-1 cells transfected with pGL3-ANLN WT/pGL3-ANLN MUT and miR-503-5p mimics/NC was determined by DLR assay. **P < 0.01 vs. the miR-NC group. D The expression of miR-503-5p in tumor tissues (n = 73) and adjacent tissues (n = 73) was detected by qRT-PCR. P < 0.01. E The correlation between miR-503-5p and ANLN. P < 0.01, r = −0.339. F The correlation between LINC00460 and ANLN. P < 0.01, r = −0.3623. G The protein level of ANLN after transfection of miR-503-5p mimics/NC into PANC-1 cells was determined by western blot assay. **P < 0.01 vs. the miR-NC group. H The protein level of ANLN in H6C7 cells and PC cell lines (ASPC-1, BxPC-3, SW1990, PANC-1 and Mia-PaCa-2) were determined by western blot assay. **P < 0.01 vs. the H6C7 cells group

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Cancer Cell International

ISSN: 1475-2867

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