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Fig. 8 | Cancer Cell International

Fig. 8

From: Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint

Fig. 8

Exosomal-LINC00460 facilitates M2 macrophage polarization. A The morphological characteristics of exosomes which was round membranous vesicle were observed by a TEM. Scale bar: 200 nm. B The levels of exosomes surface marker (TSG101, CD63 and CD81) were detected by Western blot. C The mRNA level of macrophage marker CD68 was detected by qRT-PCR. **P < 0.01 vs. the THP-1 cells group. D Uptake of exosomes was observed by a confocal microscope (400 ×). E The expression of LINC00460 in exosomes secreted by PANC-1 cells was detected by qRT-PCR. **P < 0.01 vs. the pcDNA-NC group. F The mRNA levels of M2 polarized markers (CD163, CD206, ARG1 and IL-10) were detected by qRT-PCR. *P < 0.01 vs. the control group; ##P < 0.01 vs. the Exo-pcDNA-NC group. (G) The mRNA levels of M1 polarized markers (iNOS and IL-12) were detected by qRT-PCR. *P < 0.01 vs. the control group; ##P < 0.01 vs. the Exo-pcDNA-NC group

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