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Fig. 1 | Cancer Cell International

Fig. 1

From: Establishment of anti-DKK3 peptide for the cancer control in head and neck squamous cell carcinoma (HNSCC)

Fig. 1

DKK3 deletion mutant expression plasmids and their effect on Akt phosphorylation. a Schematic explanation for the generation of DKK3 deletion mutant plasmids; DKK3ΔC1, DKK3ΔC2 and DKK3ΔC1ΔC2. The template DKK3 expression plasmid codes full-length DKK3 protein (consisting of 350 aa) with HA-tag in the COOH-terminal. Inverse PCR with primer ser U1 and D1, U2 and D2, and U1 and D2 resulted in the expression plasmid of DKK3ΔC1 (300 aa), DKK3ΔC2 (284 aa) and DKK3ΔC1ΔC2 (223aa), respectively. atg: start codon, SP: signal peptide, HA: HA-Tag. CRD1: cysteine rich domain1 (DKK-type Cys-1), CRD2: cysteine rich domain 2 (DKK-type Cys-2). (b) Amino acid sequences of DKK3ΔC1, DKK3ΔC2, and DKK3ΔC1ΔC2. b DKK3 expression is significantly elevated in DKK3 plasmid and DKK3 deletion mutant plasmids. Note that the band size was changed because of deletion and the band was shifted. c Akt expression is significantly elevated in DKK3ΔC2 and DKK3ΔC1ΔC2 transfectants. Phosphorylated Akt (p-Akt) was significantly elevated in DKK3 and DKK3ΔC2 transfectants, while it was significantly decreased in DKK3ΔC1ΔC2 transfectant compared to that in all groups

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