Fig. 1
From: Activation of embryonic/germ cell-like axis links poor outcomes of gliomas
Experimental hypothesis and the crucial role of germ cell-like cell formation in tumour initiation. A Experimental hypothesis. B Immunofluorescence assays showed the expression and colocalization of the indicated antigens in cultured U251 cells. C AP staining showing PGC-like cells in U251, LN229, A172wt and A172mut glioma cells. D The percentage of AP-positive cells in glioma cultured cells. E Survival curve of tumour-free glioma cells showing their different tumour initiation abilities. F Image of tumour tissues (HE staining) from grafted mice. G The comparison of indicated gene expression and tumour initiating ability between A172 bottom cells and A172 upper cells. (H) The efficiency of generating clones of PGC-like cells from single glioma cells. I The percentage of AP-positive cells in the control and DAZL+/− glioma cells. J The Survival curve of tumour-free cells showing the tumour initiation ability of the control and DAZL+/− glioma cells. None of the DAZL+/− cell lines could cause tumours within 100 days after grafting in nude mice. Scale bar = 20 μm in (F), 50 μm in (B, C). **P < 0.01