Fig. 4

HPV16 E5, c-Rel and miR-133a-3p all affect cell proliferation, apoptosis, migration, invasion and autophagy. A: EdU staining indicating C33-A cell proliferation increases when HPV16 E5 is overexpressed; EdU staining indicating SiHa cell proliferation decreases when Rel is silenced and when miR-133a-3p is overexpressed; B: flow cytometry analysis of annexin V and propidium iodide-stained C33-A cells transfected with HPV16 E5 OE or SiHa cells treated Rel shRNA,miR-133a-3p mimics; C:The colony-formation ability of SiHa cells with shHPV16E5,shRel and miR-133a-3p mimics compared with negative control from 50 to 200 cell inoculation densities, the colony-formation ability have all been reduced