Fig. 4
From: NRP1 inhibition modulates radiosensitivity of medulloblastoma by targeting cancer stem cells
Effect of MR438 on the expression of stem cell markers and tumors spheres number of medulloblastoma stem-like cells after tumors dissociation. Representative images of CD15 expression in tumors after irradiation and/or MR438 treatments A; scale bars represent 100 µm. The percentage of cells positive for CD15 B was calculated with QuPath open software from approximately 5000 cells for 3 different images with wide field of observation from 3 different tumors. The gene ex-pression of CD133 C and Sox2 D was analyzed by quantitative reverse transcription PCR (qRT-PCR). Tumor spheres number of medulloblastoma stem cells cultivated in serum free condition after tumors dissociation of DAOY, D283-Med and D341-Med tumors E. The analysis were performed at the experimental endpoint (corresponding to an average of 19 ± 3 days for mice of non-irradiated groups and 51 ± 8 days for mice of irradiated groups). *p < 0.05 versus CTL and #p < 0.05 versus RT, n = 6