Fig. 6

Pull-down of GST-APC^2539–2772 containing the EB1 domain. GST-APC^2539–2772 was tested against the following MBP-tagged proteins: JUN^100–331 (lane 1), JUN^FL (lane 2), FOSL1^40–282 (lane 3), TCF7^152–359 (lane 4), TCF7^FL (lane 5), and MBP alone (negative control; lane 6). The signal of MBP alone in all the samples (lanes 1–5) represents the binding of cleaved MBP protein to the amylose resin, likely resulting from leaky expression or cleavage during purification. The TCF7^152–359 and TCF7^FL constructs show fragmentation represented by multiple bands below the expected target protein. An arrow indicates the expected molecular weight of the target protein/fragment (green signal GST; red signal MBP)