Fig. 4
Microscopic analysis of B16F10 luc2 or WT31 melanoma metastases in Lyve-1−/− and Ctrl mice. A Images of H&E stainings of hepatic metastases of B16F10 luc2 melanoma in Ctrl (nmet = 25, n = 7) and Lyve-1−/− (nmet = 18, n = 5) livers. Images of Sirius red stainings of hepatic metastases of B16F10 luc2 melanoma in Ctrl (nmet = 22, n = 8) and Lyve-1−/− (nmet = 12, n = 4) livers. Scale bars: 100 µm. B Images of H&E stainings of hepatic metastases of WT31 melanoma in Ctrl (nmet = 18, n = 6) and Lyve-1−/− (nmet = 13, n = 7) livers. Images of Sirius red stainings of hepatic metastases of WT31 melanoma in Ctrl (nmet = 14, n = 5) and Lyve-1−/− (nmet = 10, n = 7) livers. Scale bars: 100 µm. C The size of liver metastases of B16F10 luc2 melanoma in Ctrl (nmet = 25, n = 7) and Lyve-1−/− (nmet = 18, n = 5) livers was measured (P = 0.0467, Mann-Whitney U-test) and area is presented in mm2. H&E stainings of hepatic B16F10 luc2 melanoma metastases were evaluated for necrosis. The percentage of necrotic metastases referred to the total number of metastases was analyzed in Ctrl (nmet = 24, n = 6) and Lyve-1−/− (nmet = 17, n = 5) (P = 0.3281, Mann-Whitney U-test). D The size of liver metastases of WT31 melanoma in Ctrl (nmet = 18, n = 6) and Lyve-1−/− (nmet = 13, n = 7) livers was measured (P = 0.9528, Mann-Whitney U-test). The area is presented in mm2. H&E stainings of hepatic WT31 melanoma metastases were evaluated for necrosis. The percentage of necrotic metastases in relation to the total number of metastases in Ctrl (nmet = 18, n = 6) and Lyve-1−/− (nmet = 13, n = 7) is presented (P = 0.6830, Mann-Whitney U-test). E Immunofluorescence stainings of hepatic B16F10 luc2 melanoma metastases for Emcn and Lyve-1 in Ctrl (nmet = 20, n = 7) and Lyve-1−/− (nmet = 17, n = 6) mice as well as for CD31 and CD32b in Ctrl (nmet = 12, n = 4) and Lyve-1−/− (nmet = 6, n = 5) mice. Scale bars: 100 µm. F Immunofluorescence stainings of hepatic WT31 melanoma metastases for Emcn and Lyve-1 in Ctrl (nmet = 14, n = 5) and Lyve-1−/− (nmet = 14, n = 7) mice as well as for CD31 and CD32b in Ctrl (nmet = 12, n = 7) and Lyve-1−/− (nmet = 7, n = 5) mice. Scale bars: 100 µm. Data information: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns not significant