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Fig. 5 | Cancer Cell International

Fig. 5

From: Lyve-1 deficiency enhances the hepatic immune microenvironment entailing altered susceptibility to melanoma liver metastasis

Fig. 5

Analysis of the immune cell composition in livers of Ctrl and Lyve-1−/− mice without metastases or with melanoma metastases of B16F10 luc2 or WT31. A Flow cytometric analysis of liver immune cells including CD4+ T cells (P = 0.0094), CD8+ T cells (P = 0.0449), Treg cells (P = 0.0389), macrophages (P = 0.7984, Mann–Whitney U-test), inflammatory monocytes (P = 0.7984, Mann–Whitney U-test), eosinophils (P = 0.0139) and neutrophils (P = 0.4159) was performed of whole untreated, tumor-free Ctrl and Lyve-1−/− livers (female, n = 8/group). B Flow cytometric analysis of whole liver tissue with B16F10 luc2 melanoma metastases of Ctrl and Lyve-1−/− mice (female, n = 8/group) for CD4+ T cells (P = 0.2381), CD8+ T cells (P = 0.8248), Treg cells (P = 0.0368), macrophages (P = 0.7209, Mann–Whitney U-test), inflammatory monocytes (P = 0.8420), eosinophils (P = 0.2228) and neutrophils (P = 0.0261). Analysis was performed on day 14. C Flow cytometric analysis of whole liver tissue with WT31 melanoma metastases of Ctrl and Lyve-1−/− mice (female, n = 8/group) for CD4+ T cells (P = 0.6411), CD8+ T cells (P = 0.6405), Treg cells (P = 0.2402), macrophages (P = 0.5676), inflammatory monocytes (P = 0.7209, Mann–Whitney U-test), eosinophils (P = 0.7984, Mann–Whitney U-test) and neutrophils (P = 0.2786, Mann–Whitney U-test). Analysis was performed on day 19. Data information: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns not significant

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