Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: METTL3 promotes glycolysis and cholangiocarcinoma progression by mediating the m6A modification of AKR1B10

Fig. 2

METTL3 promotes glycolysis and the malignant phenotype of CCA cells in vitro. A METTL3 mRNA expression in HCCC-9810 cells and RBE cells was detected by RT‒qPCR. B METTL3 protein expression in HCCC-9810 cells and RBE cells was detected by western blot. C The knockdown efficacy of two siRNAs against METTL3 mRNA expression in HCCC-9810 cells was confirmed by RT‒qPCR. D The knockdown efficacy of two siRNAs against METTL3 protein expression in HCCC-9810 cells was confirmed by western blot. E The proliferation of HCCC-9810 cells after knockdown of METTL3 was detected by CCK8. F TUNEL staining was used to detect HCCC-9810 cell apoptosis after METTL3 knockdown. Scale bar: 50 μm. G The migration and invasion of HCCC-9810 cells after METTL3 knockdown were detected by Transwell assays. The numbers of migrating and invading cells were counted for comparison. Scale bar: 100 μm. H Glucose uptake of HCCC-9810 cells after knockdown of METTL3 was detected using a commercial kit. I Lactate production in HCCC-9810 cells after knockdown of METTL3. **Means P < 0.01, ***means P < 0.001

Back to article page