Fig. 5

Gene expression profiling of NIM1-TIMP3 clone. A Enrichment plots of selected gene sets that were significantly down-regulated in NIM-T23 compared to control NIM1-EV cells. NES, NOM p-value and FDR q-value are reported for each plot. B Cnetplot of top 30 GO-BP terms enriched in 290 differentially expressed genes with a FC of at least 2 in absolute value in NIM1-T23 cells. C qRT-PCR analysis of CCL2, CXCL8, CD274 and IL-1α genes with a customized TaqMan Low Density Array. Values are presented as relative quantity (RQ Log10) normalized for HPRT-1 housekeeping gene expression. Data are normalized as ratio to the corresponding control, considered NIM1-EV = 0 as baseline and represent the mean of two independent experiments