Fig. 3
AGR2 expression in drug-exposed surviving cells. A A schematic diagram showing the timing of AGR2 detection following drug treatment. B Clonogenic growth evaluations of the primary cell lines treated with taxol, irinotecan, cisplatin, MKC8866 or etoposide. Error bars represent errors between counts for three independent experiments. Passage numbers for both cell lines ranged from 17 to 33. Three asterisks indicate significant difference at p < 0.001. C Immunofluorescence images for treated cells at IC50 values for the tested drugs. The Jed66_GB cell line was treated with taxol (42ρM), irinotecan (81 nM), cisplatin (4 μM), MKC8866 (7.4 μM) or etoposide (14 μM). The Jed41_GB cell line was treated with taxol (2 nM), irinotecan (210 nM), cisplatin (12 μM), MKC8866 (6.7 μM) or etoposide (29 μM). AGR2 is shown in red and DAPI in blue. All images were taken at 20 × magnification