Fig. 2

Modulation of HOTAIR expression in PTC cells regulates proliferation, colony formation and migration. A HOTAIR knockdown in the MDA-T32 cell line (si-HOTAIR cell) was achieved via adding HOTAIR siRNA and scramble siRNA was used to generate the Scramble cell (n = 3). B, C Cell proliferation rate of Scramble and si-HOTAIR cells was assessed by CCK-8 and colony formation assay (n = 3). D Cell migration of Scramble and si-HOTAIR cells was evaluated using wound-healing assay (n = 3). E Constitutive HOTAIR overexpression in the MDA-T41 cell line (HOTAIR-OE cell) was generated via lentiviral transduction and empty vector was utilized to generate the Control cell (n = 3). F, G Cell proliferation rate of Control and HOTAIR-OE cells was assessed by CCK-8 and colony formation assay (n = 3). H Cell migration of Control and HOTAIR-OE cells was evaluated using wound-healing assay (n = 3). Data are presented as means ± SD. Statistical significance was assessed using independent Student T-test. *p < 0.05; **p < 0.01; ***p < 0.001