Fig. 3

Modulation of HOTAIR expression in PTC cells regulates cell cycle. A Flow cytometry was performed among Scramble and si-HOTAIR cells to evaluate HOTAIR knockdown-mediated effects on cell cycle distribution (n = 3). B Protein markers of different cell phases were blotted on Scramble and si-HOTAIR cells (n = 3). C Flow cytometry was performed among Control and HOTAIR-OE cells to evaluate HOTAIR overexpression-mediated effects on cell cycle distribution (n = 3). D Protein markers of different cell phases were blotted on Control and HOTAIR-OE cells (n = 3). E PTEN and p-AKT/AKT ratio were blotted on Scramble/si-HOTAIR and Control/HOTAIR-OE cells to quantify their relative protein levels after regulating HOTAIR expression (n = 3). Data are presented as means ± SD. Statistical significance was assessed using independent Student T-test. *p < 0.05; **p < 0.01; ***p < 0.001