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Fig. 3 | Cancer Cell International

Fig. 3

From: Targeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma

Fig. 3

Effect of SF plus MU combination on HAS3 expression and HA levels in RCC cells. A–F: RCC cells were treated with SF, MU or their combination for 48 h and analyzed for HAS3 transcript levels (A, B), and protein expression (C, D). HAS3 transcript levels were normalized to β-actin. Data: Mean ± SD (n = 3). C, D: actin as the loading control. The images are cropped for brevity. Uncropped images of the blots shown in panels C and D are provided in the Additional file 1 (Appendix). Cell CM were assayed for HA levels by HA test and normalized to cell number (E, F). Note: Data shown are for the EV transfectants of 786-O and Caki-1. HA levels in the HAS3 transfectants are shown in Fig. 4

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