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Fig. 7 | Cancer Cell International

Fig. 7

From: Targeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma

Fig. 7

Analyses of anti-invasive and anti-tumor activities of SF and MU combination in EV and HAS3 transfectants. A: RCC cell transfectants treated with the combination were analyzed for chemotactic motility (at 18 h; top panel) and for invasive activity (at 48 h; bottom panel), respectively. Data: Mean ± SD (n = 3). B: The transfectants were also analyzed for markers associated with RCC invasion/metastasis; actin serves as the loading control (B). The images are cropped for brevity. Uncropped images of the blots shown in panel B are provided in the Additional file 1 (Appendix). C–E: In an athymic xenograft model, Caki-1 tumors (EV and HAS3) were established. Starting at two-weeks, mice were treated with vehicle or SF and MU combination, until week seven; six mice per group; 2 males and 4 females. Tumor volume and animal weight were measured weekly (C, E) and tumor weight was measured at endpoint (D). Data in panels C–E: Mean ± SEM

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