Fig. 8
From: Targeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma
Analysis Caki-1 subcutaneous tumors. A: Caki-1 subcutaneous tumor histology images. Tumor photos were taken at necropsy. For these photos, tumor volume and weight data are shown in 7C and D. Three photos per group are shown. Caki-1 tumors in the vehicle (both EV and HAS3 tumors) and combination (HAS3 tumors only) show invasion into muscle and the fat layers. IHC shows HAS3, HA and Ki67 (proliferation) expression, microvessels (CD31 staining), and apoptosis indicator, cleaved caspase 3 staining; magnification: 400X. B, C: Microvessels and Ki67 positive nuclei were counted at 400X magnification to determine microvessel density (MVD) and proliferation index (Ki67 positive nuclei/high power field (HPF)), respectively. Data: Mean ± SD; n = 10. D: Extracts of two randomly chosen Caki-1 subcutaneous tumors from each treatment group were analyzed for indicated proteins; actin is the loading control. The images are cropped for brevity. Uncropped images of the blots shown in panel D are provided in the Additional file 1 (Appendix)