Fig. 1

γH2AX analysis.  A, B Tumor response to 10 Gy delivered on day 21 post-implant. A BLI total flux ratio as a function of time after irradiation of NSC11 and NSC20 tumors. Values represent the mean ± SEM. B Kaplan–Meier survival curves generated for NSC11 and NSC20 tumor-bearing mice with log-rank analysis (*p < 0.0001). For γH2AX analyses 10 Gy was delivered to mice bearing NSC11 or NSC20 tumors 40 days post-implant. C Representative matched confocal images of γH2AX in NSC11 and NSC20 cells in OB and RH as a function of time after 10 Gy (×40 magnification—orthogonal projection from confocal images, DAPI, Sox2 and γH2AX are shown in blue, green and red, respectively) with mouse cells indicated by the white asteriscs. D γH2AX expression was determined by fluorescence intensity (CTCF) at 30 min after 10 Gy in NSC11 and NSC20 tumor cells in OB and RH. Values represent the mean ± SEM, n = 3. E γH2AX foci in NSC11 and NSC20 tumor cells as a function of time after 10 Gy in OB and RH. Each point corresponds to an individual mouse with at least 100 cells analyzed in each location with lines connecting the matched OB (white circle) and RH (black circle) from the same mouse. *p-val < 0.05 refers to the mean ± SEM of OB versus RH