Fig. 2

53BP1 analysis. For 53BP1 analyses 10 Gy was delivered to mice bearing NSC11 or NSC20 tumors 40 days post-implant. A Representative matched confocal images of 53BP1 in NSC11 and NSC20 tumor cells in OB and RH as a function of time after 10 Gy (×40 magnification—orthogonal projection from confocal images, DAPI, Sox2 and 53BP1 are shown in blue, green and red, respectively). B 53BP1 expression was determined by fluorescence intensity (CTCF) at 30 min after 10 Gy in NSC11 and NSC20 tumor cells in OB and RH. Values represent the mean ± SEM, n = 3. C 53BP1 foci in NSC11 and NSC20 tumor cells as a function of time after 10 Gy in OB and RH. Each point corresponds to an individual mouse with at least 100 cells analyzed in each location with lines connecting the matched OB (white circle) and RH (black circle) from the same mouse. At 24 h after 10 Gy (n = 3), the number of foci per cells were similar between the biological replicates and consequently only two lines are visible on the graphs. *p-val < 0.05 refers to the mean ± SEM of OB versus RH