Fig. 4

Gene expression analysis of NSC11 cells located in the OB versus RH. On day 40 post-implant, RNA was extracted from NSC11 tumor tissue located in OB and RH and analyzed by RNAseq. A Heat map comparing OB and RH samples (3 samples per group). B Volcano plot of transcripts significantly (red) changed in OB compared to RH. C IPA defined molecular and cellular functions differentially expressed in NSC11 cells in OB versus the RH (all had p-values < 3.40E−09). D Representative matched images of NHEJ proteins expression in NSC11 tumor cells in OB vs. RH (DAPI, Sox2 and proteins of interest are shown in blue, green and red, respectively). E NHEJ proteins expression was determined by fluorescent intensity (CTCF fold change) in NSC11 and NSC20 GSCs in OB vs. RH. Each point corresponds to an individual mouse with at least 100 cells analyzed in each location with lines connecting the matched OB (white circle) and RH (black circle) from the same mouse. Values represent the mean ± SEM, n = 3–4. *p-val < 0.05