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Fig. 6 | Cancer Cell International

Fig. 6

From: Potential of Z-100, extracted from Mycobacterium tuberculosis strain Aoyama B, as a hot tumor inducer

Fig. 6

Abolishment of the antitumor effect of Z-100 in IL-12p40 KO mice. a Tumor growth in C3H/HeN or IL-12p40 KO mice administered saline or Z-100 (1 mg/kg) (n = 15–20). Saline or Z-100 was administered subcutaneously into the right inguinal region of female C3H/HeN mice or IL-12p40 KO mice once daily for 49 days. On the 29th day of administration of saline or Z-100, Sq-1979 cells (1 × 106 cells) were injected subcutaneously into the right flank of the mice. Tumor growth was monitored by measuring tumor size (mm2) using a caliper. Data show mean ± SE. Asterisks indicate a significant difference compared with each Saline group; ** P < 0.01, NS, not significant (repeated measures ANOVA). b IL-12p40 production from BMDM derived from C3H/HeN or IL-12p40 KO mice stimulated with saline or Z-100 (100 µg/mL). Bone marrow cells were cultured in the presence of GM-CSF for 7 days to enable them to differentiate into BMDMs, then seeded onto plates and pre-incubated for 30 min. Stimulation was started after pre-incubation. After 24 h, the supernatant was collected and the IL-12p40 concentration in the supernatant was determined by ELISA. Data show mean ± SE. Asterisks indicate a significant difference compared with each Saline group; *** P < 0.001 (Student’s t-test), ND, not detected

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