Fig. 2

Migration rates of HGSOC cells along disease evolution in both wound healing (A [i–iv], B [i–iii]) and Boyden chamber (A [v–viii], B [iv–vi]) assays. In the wound healing assay, multiple images were taken along the wound at 0 and 36 h and the wound width was measured 4 times per image. The difference between the initial and final wound widths was calculated and converted into percentage of wound closure. Panels A [ii–iv] and B [ii–iii] are visual representations of each cell line after 36 h, labelled with DAPI, to stain the nucleus, and Alexa Fluor-594 Phalloidin, to stain the cytoskeleton. Scale bar = 1000 µm. In the Boyden chamber assay, cells that had migrated through the insert after 30 h were counted. Panels A [vi–viii] and B [v–vi] are visual representations of migrated cells after 30 h, labelled with SYTOX green, to stain the nucleus, and Alexa Fluor-594 Phalloidin, to stain the cytoskeleton. Scale bar = 100 µm. Data shown represents the mean ± s.e.m. For panels A[i] and A[v], **P < 0.01, ***P < 0.001 compared to PEO1, and ###P < 0.001 compared to PEO6. Statistical analysis was done using one-way ANOVA followed by Bonferroni’s test. For panels B[i] and B[iv], *P < 0.05, **P < 0.01 compared to PEO14. Statistical analysis was done using unpaired student t-test