Fig. 4

The invasion rate of HGSOC cells across disease progression in a Boyden chamber assay. Boyden chamber assays were performed similarly to the migration assays but with the addition of a layer of extracellular matrix. Panels A[ii–iv] and B[ii, iii] are visual representations of invaded cells after 30 h, labelled with SYTOX green, to label the nucleus, and Alexa Fluor-594 Phalloidin to label the cytoskeleton. Scale bar = 100 µm. Data shown represents the mean ± s.e.m. For panel A[i], *P < 0.05, **P < 0.01 compared to PEO1. Statistical analysis was done using one-way ANOVA followed by Bonferroni’s test. For panel B[i] **P < 0.01 compared to PEO14. Statistical analysis was done using unpaired student t-test