Fig. 6

The adhesion rate of HGSOC cells to organotypic models, composed of fibroblasts embedded in collagen I topped with a monolayer of LP9 cells, increases along disease progression. Panels A[ii–vii] and B[ii–v] are a visual representation of HGSOC cells adhered to the LP9 monolayer after 4 h (A[ii–iv], B[ii, iii]) and 24 h (A[v–vii], B[vi–v]). HGSOC cells were incubated with Cell Tracker™ Deep Red before plating. Cells were fixed with 4% PFA and stained for calretinin (green, [mesothelial cells]) and vimentin (red, [fibroblasts]) by immunofluorescence, and DAPI, to stain the nucleus. Scale bar = 200 µm. Data shown represents the mean ± s.e.m. For panel A[i], *P < 0.05, **P < 0.01, ***P < 0.001 compared to PEO1. For panel B[i], **P < 0.01 compared to PEO14. Statistical analysis was done using two-way ANOVA followed by Bonferroni’s test