Fig. 7

HGSOC spheroids do not adhere to an organotypic model composed of fibroblasts embedded in collagen I topped with a monolayer of LP9 mesothelial cells, nor cause mesothelial cell displacement unless incubated with conditioned media. Panel A represents a brightfield image representing the morphology of PEO6 spheroids. Panel B is a visual representation of PEO6 spheroids adhered to the organotypic model after 24 h. Panel C represents PEO6 spheroids incubated with adherent PEO6 conditioned media for 24 h. Cells were fixed with 4% PFA and stained for calretinin (green, [mesothelial cells]), vimentin (red, [fibroblasts]) by immunofluorescence, and DAPI (blue), to stain the nuclei. Inset in panel B represents spheroids that are not attached (floating) when incubated in non-conditioned media. Scale bar = 200 µm