Fig. 2

Transfer of CAF-derived lipids to cancer cells via upregulated lipid transporters. A Representative fluorescence image of FAP, BODIPY, and DAPI in xenografted tumor by transplanted in nude mice. 40 × magnification. Arrowhead indicates co-localized FAP and BODIPY fluorescence. Scale bar: 75 μm. B Profile of fatty acids content in cell pellets of primary cultured NF and CAF.1–4. ^***P < 0.001, CAFs versus NF. Data represents the mean ± SEM (n = 3). C Expression of SCD and β-actin in NF and CAFs isolated from 1 to 4 individual tumor masses. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. Data indicate the mean ± SEM (n = 3). D Transcription levels of ACLY, ACAC, and FASN in NF and CAF.1 which cultured in complete medium and treated with CAY10566 (1 μm). ^***P < 0.001. Data represent the mean ± SEM (n = 4). E Fatty acids profile in H460 cells treated with complete medium, NF and CAF.1 supernatant for 1 day. ^*P < 0.05 and ^***P < 0.001. Data represents the mean ± SEM (n = 3). F Representative histogram of BODIPY staining in H460 cells incubated in complete medium with supernatant of NF or CAF.1 for one day and subsequently treated with CAY10566. The bar graph indicated the mean value of P2 populations. Error bars represent SEM (n = 3). ^***P < 0.001. G SCD protein expression and H mRNA expression of SLC27A1 and CD36 in H460 cells incubated in complete medium with supernatant of NF or CAF.1 for one day and subsequently treated with CAY10566. ^*P < 0.05 and ^***P < 0.001. Data represents mean ± SEM (n = 4). I Profile of fatty acid contents in H460 cells treated with 50 μM SSO and CAFs-supernatant for 1 day. ^*P < 0.05 and ^***P < 0.001. Data represents the mean ± SEM (n = 3)