Fig. 3

Increased SCD in glucose-starved H460 cells that were treated with OA enhances autophagic response. A Representative histogram showing BODIPY staining in H460 cells that were treated with OA in complete or glucose-deficient medium. The bar graph indicated the mean value of P3 populations. Error bars represent the SEM (n = 4). ^***P < 0.001. B Protein expression of SREBP-1 precursor, mature SREBP-1, and SCD in H460 cells treated with OA in complete or glucose-deficient medium. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. Data indicate the mean ± SEM (n = 4). C Expression of mTOR, BECN-1, LC3-I, LC3-II, and β-actin in H460 cells treated with OA in complete or glucose-deficient medium. D Representative histogram of Cyto-ID staining of H460 cells treated with OA in complete or glucose-deficient medium. The bar graph indicated the mean value. ^***P < 0.001. The results from each experiment represent the mean ± SEM (n = 3). E Expression of SCD, LC3-I, LC3-II and β-actin in H460 cells treated with OA and either 3-MA or CQ in complete or glucose-deficient medium. The bar graph indicated that the normalization of LC3-II and SCD expression by β-actin. Data represent the mean ± SEM (n = 3)