Fig. 5

Increased SCD promotes stemness via translocation of YAP into the nucleus. A Representative fluorescence image of YAP, F-actin and DAPI in H460 cells cultured with supernatant of H460, NF or CAF in complete or glucose-deficient medium for one day. 40 × magnification. Arrowhead indicates co-localization of YAP and DAPI. Scale bar: 100 μm. B Representative dot plot of flow cytometry for side population assay. H460 cells incubated with supernatant of H460, NF or CAF followed by further culture in complete or glucose-deficient medium for one day. The P6 population was established using verapamil treatment (negative control). C Representative fluorescence image of YAP, F-actin and DAPI in parental, SCD-KO and SCD-pCDH H460 cells. Arrowhead indicates co-localized fluorescence of YAP and F-actin in the nucleus. 40 × magnification. Scale bar: 100 μm. D Expression of F-actin, YAP and lamin A/C in the nucleus of parental, SCD-KO and SCD-pCDH H460 cells treated with Lat A and normalized using lamin A/C using ImageJ. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. Data represent the mean ± SEM (n = 3). E Expression of p-cofilin, F-actin, and β-actin and (F) level of ATP in parental, SCD-KO, and SCD-pCDH H460 cells treated with etomoxir. ^***P < 0.001. Data indicate the mean ± SEM (n = 9)