Fig. 1

CT26, 4T1 and LLC-1 cell viability after exposure to 10,000–100,000 ppm gNO. A Assay scheme B–D Cancer cells were exposed to 10,000–100,000 ppm gNO or untreated cells for 10 s, 1 min, or 2.5 min. Cancer cell viability was measured after an overnight incubation in XTT proliferation assay. Assay scheme, B CT26 cell viability after exposure to 10,000–100,000 ppm gNO. C 4T1 cell viability after exposure to 10,000–100,000 ppm gNO. D LLC1 cell viability after exposure to 10,000–100,000 ppm gNO. Statistical significance between the groups was determined using One-way ANOVA with Dunnett’s multiple comparison test with Non treated group serving as the control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001 E–G The percentage of cells found at different cell death stages 24 h after a 3 min exposure to air or 50,000 ppm gNO. After ON incubation, CT26, 4T1 and LLC-1 cells were assessed for cell viability. Cell viability was assessed by Annexin V-PI fluorescence analysis 24 h after exposure