Fig. 2

The local and systemic effect of UHCgNO on CT26 tumor-bearing mice. A Assay scheme. B Tumor growth curves of CT26 tumor-bearing mice (average tumor volume on treatment day 78.61 ± 43.57 mm³) treated with 20,000 ppm or 50,000 ppm gNO or nitrogen for 5 min administered intratumorally by a 23G hypodermic needle. Statistical significance between the groups was determined using T-test. 50,000 ppm gNO versus nitrogen *P = 0.04; 20,000 ppm gNO versus nitrogen P = 0.08. C The percentage of recurrence after surgery. Fourteen days post-UHCgNO treatment, all primary tumors were excised, and the recurrence rate was monitored. In C we have included all mice that had their primary tumors resected at day 14 and had a follow-up time of at least 25 days post primary tumor resection