Fig. 3

The effect of UHCgNO on CT26 tumor challenge in vivo. A The percentage of mice in which the challenge resulted in a new tumor. Chi-square test (naïve versus. 50,000 ppm gNO or 20,000 ppm gNO groups) P < 0.05 B Preliminary challenge assay where mice were treated with 50,000 ppm gNO or nitrogen for 5 min. Two weeks post gas treatment, the tumors were resected and a week later a secondary tumor was induced in the contralateral flank. Fisher’s Exact test, *P < 0.05 C Average tumor volume of the challenge tumor. One mouse in the 20,000 ppm arm was excluded from this analysis; this mouse was euthanized on Day 14 post challenge assay start due to tumor volume. D Challenge tumor take percentage following either 20,000 ppm gNO, 50,000 ppm gNO, or no treatment. Two mice in the 50,000 ppm arm were excluded from this analysis due to a handling protocol violation following treatment; all other deaths were due to tumor volume triggering euthanasia. The mouse from the 20,000 ppm NO group that was excluded from graph 3C was included in this analysis as the mouse developed a challenge tumor before exclusion from the study E Survival graph of gNO -treated and control mice, P < 0.001 Mantel Cox logrank test