Fig. 3From: Cell senescence-associated genes predict the malignant characteristics of glioblastoma Validation of PTTG1 and MYC expression and their function in glioma. A The expression level of PTTG1 and MYC in normal tissue and glioma from the HPA. B Representative images of IHC staining for PTTG1 and MYC in tumor and peritumor tissue (scale bar: 50Â mm). (C) Western blot was used to verify the efficiency of PTTG1 and MYC downregulation. D The result of EdU assay 48Â h after transfection was showed. E Representative images showing invading spheroids in the 3D invasion assay for U251 cells transfected with si-RNA and control evaluated at 24Â h, 48 and 96Â hBack to article page