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Fig. 7 | Cancer Cell International

Fig. 7

From: Increased expression of miR-194-5p through the circPVRL3/miR-194-5p/SOCS2 axis promotes proliferation and metastasis in pancreatic ductal adenocarcinoma by activating the PI3K/AKT signaling pathway

Fig. 7

SOCS2 inhibits the PDAC cell proliferation and negatively regulates the activity of PI3K/AKT signaling pathway. A, B EdU and colony formation assays were performed to detect the proliferation ability of SOCS2 in PDAC cells. Scale bar = 100 μm. C Transwell assays were performed to observe the migration of PDAC cells after treatment with SOCS2 overexpression plasmids or si-SOCS2. Scale bar = 200 μm. D The level of SOCS2 in the miR-194-5p overexpression or knockdown group was determined by RT‒qPCR. E The activation level of the PI3K/AKT signaling pathway was observed in the groups with miR-194-5p mimic and SOCS2 overexpression alone or combined and the groups with miR-194-5p inhibitor and si-SOCS2 alone or combined by western blotting. *p < 0.05; **p < 0.01; ***p < 0.001

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