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Fig. 8 | Cancer Cell International

Fig. 8

From: Increased expression of miR-194-5p through the circPVRL3/miR-194-5p/SOCS2 axis promotes proliferation and metastasis in pancreatic ductal adenocarcinoma by activating the PI3K/AKT signaling pathway

Fig. 8

CircPVRL3, as a miR-194-5p sponge, is downregulated in PDAC. A The downregulated circRNAs of GSE79634 in PDAC tissue (Gene set 1) and the potential target of miR-194-5p were predicted by CircBase (Gene set 2) and intersected to obtain the upstream gene of miR-194-5p. B The circPVRL3 information is illustrated from CircBase, and the backsplice site indicates Sanger sequencing. C WT and Mut circPVRL3 were constructed and showed the binding sites between miR-194-5p and circPVRL3. D A dual-luciferase reporter assay was performed in PDAC cells co-transfected with WT/Mut circPVRL3 plasmids and miR-194-5p mimic or mimic NC. E RIP and RT‒qPCR assays were performed to confirm the binding of miR-194-5p to the AGO2 protein. F The expression of circPVRL3 in 20 paired PDAC tissues and adjacent tissues was detected by circRNA profiling. G The co-localization of Cy3-labeled circPVRL3 and FAM-labeled miR-194-5p was detected by confocal microscopy at a magnification of 1000×. Scale bar = 5 μm

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Cancer Cell International

ISSN: 1475-2867

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