Fig. 11

H&E staining of organs from mice injected IP with parental, CRISPR control, gRNA1 and gRNA2 cells. Tumour infiltration into liver and pancreas after IP injection of parental, CRISPR control, gRNA1 and gRNA2 cell lines is demonstrated. The infiltrating front of the tumour in each case is highlighted with a black dotted line and the tumour area within each organ is indicated by T. No infiltration of tumours was observed in lungs. Top panels of each organ are ×10 magnification and black scale bar is 500 µm; bottom panels are ×40 magnification (of the square area in the ×10 image) and yellow scale bar is 75 µm. No infiltration of tumour was observed in mice injected with gRNA2 cells. However, apparent inflammatory cells in liver and lungs of mice injected with gRNA2 cells were evident and is indicated by black arrows